E-ISSN 2146-9369 | ISSN 2146-3158
 
2024, Vol: 14, Issue: 3
14 / 3Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles

Research Article 


Prevalence and molecular characterization of extended spectrum beta-Lactamases-producing uropathogenic Escherichia coli isolated in Zakho, Iraq

Reem F Polse, Samira Y Yousif, Mahde Saleh Assafi.


Cited By:36

Abstract
Objectives: Uropathogenic Escherichia coli are the most important group of microorganisms responsible for urinary tract infection. A high percentage of uropathogenic E. coli over the world are detected to be ESBLs producers, which is now a problem that limits therapeutic options. The aim of this study is to determine the prevalence of ESBLs E. coli and study the prevalence of different ESBLs genotype patterns among the ESBLs producing isolates.
Methods: One hundred and six uropathogenic E. coli isolates were analyzed for their ESBL production by molecular methods.
Results: 55 (52%) isolates were detected as extended spectrum β-lactamases (ESBLs) producers. Based on the PCR results, all E. coli isolates possessed one or more ESBL gene. CTX-M type ESBL was the most dominant ESBL (87.2%) among the isolates. While those for TEM-type and SHV-type were 54.5% and 21.8% respectively. Six genotype patterns were detected (TEM, CTX-M, TEM+SHV, TEM+CTX-M, SHV+CTX-M and TEM+SHV+CTX-M). The genotype CTX-M was the most prevalent genotype (40%) followed by the genotype TEM+CTX-M combination (30.9%). The occurrences of the genotypes (TEM, TEM+SHV, SHV+CTX-M and TEM+SHV+CTX-M) were 7.3%, 5.5%, 5.4%, 10.9%, respectively.
Conclusions: Control measures and education programs are necessary to avoid the uncontrollable use of β-lactam and cephalosporins in order to minimize the emergence of ESBLs producing E. coli. J Microbiol Infect Dis 2016;6(4): 163-167

Key words: ESBL, Uropathogenic E. coli, SHV, TEM, CTX-M, Iraq


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Reem F Polse
Articles by Samira Y Yousif
Articles by Mahde Saleh Assafi
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Reem F Polse, Samira Y Yousif, Mahde Saleh Assafi. Prevalence and molecular characterization of extended spectrum beta-Lactamases-producing uropathogenic Escherichia coli isolated in Zakho, Iraq. J Microbiol Infect Dis. 2016; 6(4): 163-167 . doi:10.5799/jmid.328863

Web Style

Reem F Polse, Samira Y Yousif, Mahde Saleh Assafi. Prevalence and molecular characterization of extended spectrum beta-Lactamases-producing uropathogenic Escherichia coli isolated in Zakho, Iraq. https://www.jmidonline.org/?mno=302657134 [Access: October 12, 2024]. doi:10.5799/jmid.328863

AMA (American Medical Association) Style

Reem F Polse, Samira Y Yousif, Mahde Saleh Assafi. Prevalence and molecular characterization of extended spectrum beta-Lactamases-producing uropathogenic Escherichia coli isolated in Zakho, Iraq. J Microbiol Infect Dis. 2016; 6(4): 163-167 . doi:10.5799/jmid.328863


Vancouver/ICMJE Style

Reem F Polse, Samira Y Yousif, Mahde Saleh Assafi. Prevalence and molecular characterization of extended spectrum beta-Lactamases-producing uropathogenic Escherichia coli isolated in Zakho, Iraq. J Microbiol Infect Dis. (2016), [cited October 12, 2024]; 6(4): 163-167 . doi:10.5799/jmid.328863


Harvard Style

Reem F Polse, Samira Y Yousif, Mahde Saleh Assafi (2016) Prevalence and molecular characterization of extended spectrum beta-Lactamases-producing uropathogenic Escherichia coli isolated in Zakho, Iraq. J Microbiol Infect Dis, 6 (4), 163-167 . doi:10.5799/jmid.328863


Turabian Style

Reem F Polse, Samira Y Yousif, Mahde Saleh Assafi. 2016. Prevalence and molecular characterization of extended spectrum beta-Lactamases-producing uropathogenic Escherichia coli isolated in Zakho, Iraq. Journal of Microbiology and Infectious Diseases, 6 (4), 163-167 . doi:10.5799/jmid.328863


Chicago Style

Reem F Polse, Samira Y Yousif, Mahde Saleh Assafi. "Prevalence and molecular characterization of extended spectrum beta-Lactamases-producing uropathogenic Escherichia coli isolated in Zakho, Iraq." Journal of Microbiology and Infectious Diseases 6 (2016), 163-167 . doi:10.5799/jmid.328863


MLA (The Modern Language Association) Style

Reem F Polse, Samira Y Yousif, Mahde Saleh Assafi. "Prevalence and molecular characterization of extended spectrum beta-Lactamases-producing uropathogenic Escherichia coli isolated in Zakho, Iraq." Journal of Microbiology and Infectious Diseases 6.4 (2016), 163-167 . Print. doi:10.5799/jmid.328863


APA (American Psychological Association) Style

Reem F Polse, Samira Y Yousif, Mahde Saleh Assafi (2016) Prevalence and molecular characterization of extended spectrum beta-Lactamases-producing uropathogenic Escherichia coli isolated in Zakho, Iraq. Journal of Microbiology and Infectious Diseases, 6 (4), 163-167 . doi:10.5799/jmid.328863


Author LoginReviewer LoginAbout PublisherOpen Access PolicyEditorial PoliciesPeer Review PolicyPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyProtection of Research Participants (Statement On Human And Animal Rights)Privacy PolicyCorrections, Retractions & Expressions of ConcernSelf-Archiving PoliciesDigital Archiving & Preservation PoliciesStatement of Informed ConsentAdvertising PolicyTerms of UseLicense InformationCopyright Information

About Journal of Microbiology and Infectious Diseases

Journal of Microbiology and Infectious Diseases (JMID) is an international "peer review" journal in microbiology, infectious diseases, virology, ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes

Editor-in-Chief

Journal of Microbiology and Infectious Diseases

Department of Microbiology and Parasitology 

Faculty of Veterinary Medicine

University of Tripoli

P.O. Box 13662, Tripoli, Libya