E-ISSN 2146-9369 | ISSN 2146-3158
 
2024, Vol: 14, Issue: 3
14 / 3Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles

Research Article 


Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa.


Cited By:2

Abstract
Objectives:Echinococcosis is a cyclozoonotic disease caused by E. granulosus. The canine mainly the dog represents the definitive host and immunity plays an important role in the control of the disease. This study was aimed to detect Echinococcus granulosus B-antibody for Dot-ELISA and antigen for counter-current immune electrophoresis (CIEP) and bacterial co-agglutination assay (Co-A).
Methods: Follow-up of 23 surgical and chemotherapy patients from different hospitals of Khartoum were performed.
Results: Results: Sensitivity and specificity for Dot-ELISA was 94.4% and 100%, respectively within seven days to 6 months except for the relapse all or most of the cases were negative for the disease whereas the sensitivity of CIEP and Co-A for Echinococcusgranulosus antigen in patient sera was 77.8% and 94.4% respectively with a specificity of 100% and 75% respectively. Dot-ELISA, showed high positive and negative predictive value (100%, 95.2%), respectively with CIEP (100%, 83.3%) and in Co-A (81%, 94.1%).
Conclusion: Conclusion: Therefore, detection of antigen of E. granulosus in the serum of operative, post-operative, or chemotherapeutic treated patient using CIEP and Co-A or detection of antibodies using IgG ELISA to the antigen B-rich fraction in (ELISA) assay is useful for following treated hydatid patients. J Microbiol Infect Dis 2021; 11(2):88-94.

Key words: Echinococcus granulosus, Dot-ELISA, Co-agglutination assay, immune electrophoresis


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Omer Hassan Siddig Elamin
Articles by Mai Abdalla Ali
Articles by Ibrahim Alhag Almahadi Elgezuli
Articles by Mona Alrasheed Bashir
Articles by Ivan Kahwa
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa. Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. J Microbiol Infect Dis. 2021; 11(2): 88-94 . doi:10.5799/jmid.951600

Web Style

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa. Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. https://www.jmidonline.org/?mno=302657119 [Access: November 23, 2024]. doi:10.5799/jmid.951600

AMA (American Medical Association) Style

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa. Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. J Microbiol Infect Dis. 2021; 11(2): 88-94 . doi:10.5799/jmid.951600


Vancouver/ICMJE Style

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa. Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. J Microbiol Infect Dis. (2021), [cited November 23, 2024]; 11(2): 88-94 . doi:10.5799/jmid.951600


Harvard Style

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa (2021) Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. J Microbiol Infect Dis, 11 (2), 88-94 . doi:10.5799/jmid.951600


Turabian Style

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa. 2021. Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. Journal of Microbiology and Infectious Diseases, 11 (2), 88-94 . doi:10.5799/jmid.951600


Chicago Style

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa. "Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination." Journal of Microbiology and Infectious Diseases 11 (2021), 88-94 . doi:10.5799/jmid.951600


MLA (The Modern Language Association) Style

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa. "Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination." Journal of Microbiology and Infectious Diseases 11.2 (2021), 88-94 . Print. doi:10.5799/jmid.951600


APA (American Psychological Association) Style

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa (2021) Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. Journal of Microbiology and Infectious Diseases, 11 (2), 88-94 . doi:10.5799/jmid.951600


Author LoginReviewer LoginAbout PublisherOpen Access PolicyEditorial PoliciesPeer Review PolicyPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyProtection of Research Participants (Statement On Human And Animal Rights)Privacy PolicyCorrections, Retractions & Expressions of ConcernSelf-Archiving PoliciesDigital Archiving & Preservation PoliciesStatement of Informed ConsentAdvertising PolicyTerms of UseLicense InformationCopyright Information

About Journal of Microbiology and Infectious Diseases

Journal of Microbiology and Infectious Diseases (JMID) is an international "peer review" journal in microbiology, infectious diseases, virology, ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes

Editor-in-Chief

Journal of Microbiology and Infectious Diseases

Department of Microbiology and Parasitology 

Faculty of Veterinary Medicine

University of Tripoli

P.O. Box 13662, Tripoli, Libya