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Research Article 


Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs

Mustafa Altındiş, Zafer Çetinkaya, Raike Kalaycı, İhsan H. Çiftçi, Alpaslan Arslan, Orhan C. Aktepe.


Abstract
Objectives: The aim of the present study was to evaluate the efficacy (recovery rate, time to detection and Drug Susceptibility Tests –DST- of Mycobacteria-only B460) of new colorimetric medium, Dio-TK and to compare it with routinely used conventional media, Lowenstein Jensen (LJ) and Bactec 460 TB culture system.
Materials and methods: Totally 901 clinic specimens were investigated for assignment of tuberculosis by Ehrlich-Ziehl-Nielsen smear strain method, Lowenstein-Jensen, BACTEC 460TB and Dio-TK medium culture systems.
Results: Nineteen of 901 clinic specimens (2.1%) were positive by any of these methods. 17 (89.5%) of these specimens positive found by smear strain method, 17 (89.5%) by Lowenstein-Jensen, 19 (100%) by BACTEC 460TB and 14 (73.7%) by Dio-TK medium. NAP and Niacin identification tests were applied to Mycobacterium strains. 12 (63.1%) of 19 isolates were identified as M.tuberculosis complex and 7 (36.9%) were identified as Mycobacterium other than tuberculosis (MOTT) bacilli. 10 (83.3%) of 12 M.tuberculosis complex strains were not resistant to any major drug. But one of 2 isolate was resistant to streptomycin and the other one isolate was resistant to both streptomycin and isoniazid.
Conclusion: Our data suggest that some advantages (such as an early detection and differentiation mycobacterium growth from contamination) of the Dio-TK CS over other mycobacterial culture systems make it a practical and rapid system for daily use, and a suitable alternative to other currently available solid media, such as LJ, for detection time of mycobacteria and DST. J Microbiol Infect Dis 2011;1(1):5-9. doi: 10.5799/ahinjs.02.2011.01.0002

Key words: Dio-TK medium, Mycobacterium tuberculosis, detection, resistance


 
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How to Cite this Article
Pubmed Style

AltındiÅŸ M, Cetinkaya Z, Kalaycı R, Ciftci ÄH, Arslan A, Aktepe OC. Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs. J Microbiol Infect Dis. 2011; 1(1): 5-9 . doi:10.5799/jmid2011.01.0002

Web Style

AltındiÅŸ M, Cetinkaya Z, Kalaycı R, Ciftci ÄH, Arslan A, Aktepe OC. Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs. https://www.jmidonline.org/?mno=302657098 [Access: April 10, 2025]. doi:10.5799/jmid2011.01.0002

AMA (American Medical Association) Style

AltındiÅŸ M, Cetinkaya Z, Kalaycı R, Ciftci ÄH, Arslan A, Aktepe OC. Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs. J Microbiol Infect Dis. 2011; 1(1): 5-9 . doi:10.5799/jmid2011.01.0002


Vancouver/ICMJE Style

AltındiÅŸ M, Cetinkaya Z, Kalaycı R, Ciftci ÄH, Arslan A, Aktepe OC. Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs. J Microbiol Infect Dis. (2011), [cited April 10, 2025]; 1(1): 5-9 . doi:10.5799/jmid2011.01.0002


Harvard Style

AltındiÅŸ, M., Cetinkaya, . Z., Kalaycı, . R., Ciftci, . Ä. H., Arslan, . A. & Aktepe, . O. C. (2011) Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs. J Microbiol Infect Dis, 1 (1), 5-9 . doi:10.5799/jmid2011.01.0002


Turabian Style

Altındiş, Mustafa, Zafer Cetinkaya, Raike Kalaycı, İhsan H. Ciftci, Alpaslan Arslan, and Orhan C. Aktepe. 2011. Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs. Journal of Microbiology and Infectious Diseases, 1 (1), 5-9 . doi:10.5799/jmid2011.01.0002


Chicago Style

Altındiş, Mustafa, Zafer Cetinkaya, Raike Kalaycı, İhsan H. Ciftci, Alpaslan Arslan, and Orhan C. Aktepe. "Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs." Journal of Microbiology and Infectious Diseases 1 (2011), 5-9 . doi:10.5799/jmid2011.01.0002


MLA (The Modern Language Association) Style

Altındiş, Mustafa, Zafer Cetinkaya, Raike Kalaycı, İhsan H. Ciftci, Alpaslan Arslan, and Orhan C. Aktepe. "Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs." Journal of Microbiology and Infectious Diseases 1.1 (2011), 5-9 . Print. doi:10.5799/jmid2011.01.0002


APA (American Psychological Association) Style

AltındiÅŸ, M., Cetinkaya, . Z., Kalaycı, . R., Ciftci, . Ä. H., Arslan, . A. & Aktepe, . O. C. (2011) Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs. Journal of Microbiology and Infectious Diseases, 1 (1), 5-9 . doi:10.5799/jmid2011.01.0002


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Journal of Microbiology and Infectious Diseases

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University of Tripoli

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