E-ISSN 2146-9369 | ISSN 2146-3158
 
2024, Vol: 14, Issue: 1
14 / 1Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles

Research Article 


Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea

Ferdaus Mohd Altaf Hossain, Parvez Khan, Iman Zahran, Shaikh Muhammad Atif, Mohammad Ali Zinnah, Imdad Ullah Khan, Md Masudur Rahman, Farzana Islam Sumaiya.


Abstract
Objectives: The study was aimed to evaluate the roles of Lactobacillus acidophilus supplementation in maintaining intestinal epithelial integrities, tight junction proteins, and adhesion molecules in non-specific diarrhea.<br>Methods: In this study, we used the pre-weaned BL/6 pups (3 weeks of age, same litters) as a model animal. We supplied the non-sterilized and poor-quality water to experimental pups (n=7) to develop non-specific diarrheic symptoms. Then diarrheic pups were supplemented with Lactobacillus acidophilus (LA) for three consecutive weeks. The control group (n=5) was supplied with sterilized water and no LA. The sampling and analysis were performed on day 0, day 7, day 14, and day 21. The expressions of pro-inflammatory cytokines (IL-6, TNF-a) and tight junction proteins (TJPs) of gut mucosa were determined using qRT-PCR. And the serum cytokines level was screened through sandwich ELISA.<br>Results: The intestinal cytoskeletal integrity becomes disrupted and characterized by lower ZO-1, Occludin, Claudin-1, Claudin-5, and JAM mRNA expressions upon real-time qRT-PCR. However, Claudin-4 was found to be not affected and illustrated with a higher expression like control pups. Interestingly, supplementing Lactobacillus acidophilus was found to maintain gut integrity and effectively reduce diarrheic symptoms. Like the control pups, the Lactobacillus acidophilus supplemented pups exhibited a higher expression of gut epithelial TJPs and adhesion molecules. Moreover, the diseased pups produced significantly increased IL-6, and TNF-α production in blood serum, compared to control BL/6 pups.<br>Conclusion: We concluded that L. acidophilus supplementation might orchestrate the equilibrium of gut health and immunity against non-specific diarrhea. J Microbiol Infect Dis 2022; 12(2):54-62.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Ferdaus Mohd Altaf Hossain
Articles by Parvez Khan
Articles by Iman Zahran
Articles by Shaikh Muhammad Atif
Articles by Mohammad Ali Zinnah
Articles by Imdad Ullah Khan
Articles by Md Masudur Rahman
Articles by Farzana Islam Sumaiya
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Ferdaus Mohd Altaf Hossain, Parvez Khan, Iman Zahran, Shaikh Muhammad Atif, Mohammad Ali Zinnah, Imdad Ullah Khan, Md Masudur Rahman, Farzana Islam Sumaiya. Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea. J Microbiol Infect Dis. 2022; 12(2): 54-62 . doi:10.5799/jmid.1130109

Web Style

Ferdaus Mohd Altaf Hossain, Parvez Khan, Iman Zahran, Shaikh Muhammad Atif, Mohammad Ali Zinnah, Imdad Ullah Khan, Md Masudur Rahman, Farzana Islam Sumaiya. Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea. https://www.jmidonline.org/?mno=302657032 [Access: April 27, 2024]. doi:10.5799/jmid.1130109

AMA (American Medical Association) Style

Ferdaus Mohd Altaf Hossain, Parvez Khan, Iman Zahran, Shaikh Muhammad Atif, Mohammad Ali Zinnah, Imdad Ullah Khan, Md Masudur Rahman, Farzana Islam Sumaiya. Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea. J Microbiol Infect Dis. 2022; 12(2): 54-62 . doi:10.5799/jmid.1130109


Vancouver/ICMJE Style

Ferdaus Mohd Altaf Hossain, Parvez Khan, Iman Zahran, Shaikh Muhammad Atif, Mohammad Ali Zinnah, Imdad Ullah Khan, Md Masudur Rahman, Farzana Islam Sumaiya. Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea. J Microbiol Infect Dis. (2022), [cited April 27, 2024]; 12(2): 54-62 . doi:10.5799/jmid.1130109


Harvard Style

Ferdaus Mohd Altaf Hossain, Parvez Khan, Iman Zahran, Shaikh Muhammad Atif, Mohammad Ali Zinnah, Imdad Ullah Khan, Md Masudur Rahman, Farzana Islam Sumaiya (2022) Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea. J Microbiol Infect Dis, 12 (2), 54-62 . doi:10.5799/jmid.1130109


Turabian Style

Ferdaus Mohd Altaf Hossain, Parvez Khan, Iman Zahran, Shaikh Muhammad Atif, Mohammad Ali Zinnah, Imdad Ullah Khan, Md Masudur Rahman, Farzana Islam Sumaiya. 2022. Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea. Journal of Microbiology and Infectious Diseases, 12 (2), 54-62 . doi:10.5799/jmid.1130109


Chicago Style

Ferdaus Mohd Altaf Hossain, Parvez Khan, Iman Zahran, Shaikh Muhammad Atif, Mohammad Ali Zinnah, Imdad Ullah Khan, Md Masudur Rahman, Farzana Islam Sumaiya. "Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea." Journal of Microbiology and Infectious Diseases 12 (2022), 54-62 . doi:10.5799/jmid.1130109


MLA (The Modern Language Association) Style

Ferdaus Mohd Altaf Hossain, Parvez Khan, Iman Zahran, Shaikh Muhammad Atif, Mohammad Ali Zinnah, Imdad Ullah Khan, Md Masudur Rahman, Farzana Islam Sumaiya. "Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea." Journal of Microbiology and Infectious Diseases 12.2 (2022), 54-62 . Print. doi:10.5799/jmid.1130109


APA (American Psychological Association) Style

Ferdaus Mohd Altaf Hossain, Parvez Khan, Iman Zahran, Shaikh Muhammad Atif, Mohammad Ali Zinnah, Imdad Ullah Khan, Md Masudur Rahman, Farzana Islam Sumaiya (2022) Lactobacillus acidophilus supplementation re-establishes gut epithelial integrities and barrier functions in non-specific diarrhea. Journal of Microbiology and Infectious Diseases, 12 (2), 54-62 . doi:10.5799/jmid.1130109


Author LoginReviewer LoginAbout PublisherOpen Access PolicyEditorial PoliciesPeer Review PolicyPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyProtection of Research Participants (Statement On Human And Animal Rights)Privacy PolicyCorrections, Retractions & Expressions of ConcernSelf-Archiving PoliciesDigital Archiving & Preservation PoliciesStatement of Informed ConsentAdvertising PolicyTerms of UseLicense InformationCopyright Information

About Journal of Microbiology and Infectious Diseases

Journal of Microbiology and Infectious Diseases (JMID) is an international "peer review" journal in microbiology, infectious diseases, virology, ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes

Editor-in-Chief

Journal of Microbiology and Infectious Diseases

Department of Microbiology and Parasitology 

Faculty of Veterinary Medicine

University of Tripoli

P.O. Box 13662, Tripoli, Libya