E-ISSN 2146-9369 | ISSN 2146-3158
 
2024, Vol: 14, Issue: 3
14 / 3Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles

Research Article


Assessment of Listeria monocytogenes contamination and antibiotic resistance in raw milk samples collected from Colombo district Sri Lanka

Hewa Babarandage Chathurika Harshani, Dilshani Kavindya Balasuriya, Wickramasinghe Widana Pathirana Pragathi Nirasha Diwyanjalee, Aranwela Gamage Gaya Kaushalya, Wijendra Acharige Somalatha Wijendra.


Abstract
Background:
Listeria monocytogenes, a foodborne pathogen, poses a significant risk to public health due to its potential to cause severe infections termed “listeriosis”. Raw milk and dairy products are common vehicles for L. monocytogenes transmission, amplifying the risk to consumers. Additionally, the emergence of antibiotic resistance in Listeria strains heightens concerns in human and veterinary medicine.
Aim:
This study aimed to assess the prevalence of L. monocytogenes in raw milk samples from Sri Lanka’s Colombo district and to characterize the antibiotic resistance patterns of positive isolates.
Methods:
One hundred raw milk samples were collected from various milk collection centers. Samples were sourced from locations including Awissawella, Kahanthota, Meepe, Homagama, and Udahamulla. Antibiotic resistance was evaluated using standard methods like disk diffusion and well diffusion, testing ampicillin, penicillin, streptomycin, and tetracycline.
Results:
Of the 100 samples, L. monocytogenes was detected by conventional PCR in 83, with Awissawella having the highest occurrence (100%), followed by Kahanthota (81.81%), Meepe (81.48%), Homagama (77.27%), and Udahamulla (44.44%). Among the positive samples, 72 showed varying degrees of antibiotic resistance. The highest level of resistance was observed for penicillin (83.1%), followed by ampicillin (65%), streptomycin (32.53%), and tetracycline (10.84%). Sixty isolates (60%) displayed resistance to more than one antibiotic studied. Findings also revealed the widespread manual milking, predominant water usage over soap for hygiene, and common daily bathing of cows, highlighting the need for improved hygiene practices to minimize contamination risks in dairy farming.
Conclusion:
Raw milk contaminated with L. monocytogenes pose a serious public health risk. The study highlights the need for stringent hygiene measures in milk production and processing to safeguard consumers. Additionally, the high prevalence of antibiotic-resistant strains underscores the importance of judicious antibiotic use in both human and veterinary medicine to mitigate the further emergence and spread of resistant Listeria strains.

Key words: Antibiotic resistance, Listeria monocytogenes, Raw milk, PCR, Antibiotic Susceptibility Testing


 
ARTICLE TOOLS
Abstract
PDF Fulltext
HTML Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Hewa Babarandage Chathurika Harshani
Articles by Dilshani Kavindya Balasuriya
Articles by Wickramasinghe Widana Pathirana Pragathi Nirasha Diwyanjalee
Articles by Aranwela Gamage Gaya Kaushalya
Articles by Wijendra Acharige Somalatha Wijendra
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Harshani HBC, Balasuriya DK, , Kaushalya AGG, Wijendra WAS. Assessment of Listeria monocytogenes contamination and antibiotic resistance in raw milk samples collected from Colombo district Sri Lanka. J Microbiol Infect Dis. 2024; 14(2): 67-79. doi:10.5455/JMID.2024.v14.i2.5

Web Style

Harshani HBC, Balasuriya DK, , Kaushalya AGG, Wijendra WAS. Assessment of Listeria monocytogenes contamination and antibiotic resistance in raw milk samples collected from Colombo district Sri Lanka. https://www.jmidonline.org/?mno=194248 [Access: October 12, 2024]. doi:10.5455/JMID.2024.v14.i2.5

AMA (American Medical Association) Style

Harshani HBC, Balasuriya DK, , Kaushalya AGG, Wijendra WAS. Assessment of Listeria monocytogenes contamination and antibiotic resistance in raw milk samples collected from Colombo district Sri Lanka. J Microbiol Infect Dis. 2024; 14(2): 67-79. doi:10.5455/JMID.2024.v14.i2.5


Vancouver/ICMJE Style

Harshani HBC, Balasuriya DK, , Kaushalya AGG, Wijendra WAS. Assessment of Listeria monocytogenes contamination and antibiotic resistance in raw milk samples collected from Colombo district Sri Lanka. J Microbiol Infect Dis. (2024), [cited October 12, 2024]; 14(2): 67-79. doi:10.5455/JMID.2024.v14.i2.5


Harvard Style

Harshani, H. B. C., Balasuriya, . D. K., , Kaushalya, . A. G. G. & Wijendra, . W. A. S. (2024) Assessment of Listeria monocytogenes contamination and antibiotic resistance in raw milk samples collected from Colombo district Sri Lanka. J Microbiol Infect Dis, 14 (2), 67-79. doi:10.5455/JMID.2024.v14.i2.5


Turabian Style

Harshani, Hewa Babarandage Chathurika, Dilshani Kavindya Balasuriya, Wickramasinghe Widana Pathirana Pragathi Nirasha Diwyanjalee, Aranwela Gamage Gaya Kaushalya, and Wijendra Acharige Somalatha Wijendra. 2024. Assessment of Listeria monocytogenes contamination and antibiotic resistance in raw milk samples collected from Colombo district Sri Lanka. Journal of Microbiology and Infectious Diseases, 14 (2), 67-79. doi:10.5455/JMID.2024.v14.i2.5


Chicago Style

Harshani, Hewa Babarandage Chathurika, Dilshani Kavindya Balasuriya, Wickramasinghe Widana Pathirana Pragathi Nirasha Diwyanjalee, Aranwela Gamage Gaya Kaushalya, and Wijendra Acharige Somalatha Wijendra. "Assessment of Listeria monocytogenes contamination and antibiotic resistance in raw milk samples collected from Colombo district Sri Lanka." Journal of Microbiology and Infectious Diseases 14 (2024), 67-79. doi:10.5455/JMID.2024.v14.i2.5


MLA (The Modern Language Association) Style

Harshani, Hewa Babarandage Chathurika, Dilshani Kavindya Balasuriya, Wickramasinghe Widana Pathirana Pragathi Nirasha Diwyanjalee, Aranwela Gamage Gaya Kaushalya, and Wijendra Acharige Somalatha Wijendra. "Assessment of Listeria monocytogenes contamination and antibiotic resistance in raw milk samples collected from Colombo district Sri Lanka." Journal of Microbiology and Infectious Diseases 14.2 (2024), 67-79. Print. doi:10.5455/JMID.2024.v14.i2.5


APA (American Psychological Association) Style

Harshani, H. B. C., Balasuriya, . D. K., , Kaushalya, . A. G. G. & Wijendra, . W. A. S. (2024) Assessment of Listeria monocytogenes contamination and antibiotic resistance in raw milk samples collected from Colombo district Sri Lanka. Journal of Microbiology and Infectious Diseases, 14 (2), 67-79. doi:10.5455/JMID.2024.v14.i2.5


Author LoginReviewer LoginAbout PublisherOpen Access PolicyEditorial PoliciesPeer Review PolicyPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyProtection of Research Participants (Statement On Human And Animal Rights)Privacy PolicyCorrections, Retractions & Expressions of ConcernSelf-Archiving PoliciesDigital Archiving & Preservation PoliciesStatement of Informed ConsentAdvertising PolicyTerms of UseLicense InformationCopyright Information

About Journal of Microbiology and Infectious Diseases

Journal of Microbiology and Infectious Diseases (JMID) is an international "peer review" journal in microbiology, infectious diseases, virology, ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes

Editor-in-Chief

Journal of Microbiology and Infectious Diseases

Department of Microbiology and Parasitology 

Faculty of Veterinary Medicine

University of Tripoli

P.O. Box 13662, Tripoli, Libya