E-ISSN 2146-9369 | ISSN 2146-3158
 
2024, Vol: 14, Issue: 1
14 / 1Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles

Research Article 


MecA and ermA Gene Discrepancy from Their Phenotypic Profile in Staphylococcus aureus Isolates

Shreya Mahesh, RajKumar Kalyan, Prashant Gupta, Sheetal Verma, Vimala Venkatesh, Piyush Tripathi.

Abstract
Objectives: Staphylococcus aureus is one of the most important bacterial pathogens in clinical practice and a primary diagnostic focus for the routine microbiology laboratory. The aim of this study was to find out the phenotypic and genotypic variations in Staphylococcus aureus isolates at a tertiary care center in Lucknow.<br>Methods: 140 clinical isolates of S. aureus were taken in the study. Kirby–Bauer disc diffusion method was performed to identify antibiotic susceptibility testing, phenotypically methicillin-resistant Staphylococcus aureus (MRSA) were identified by using cefoxitin disc (30 μg), and inducible clindamycin resistance was identified by the presence of D-shaped zone around clindamycin and by using conventional PCR method mecA and ermA genes were identified.<br>Results: Out of 140 clinical isolates S. aureus, 93 (66.4%) were MRSA, and 47 (33.6%) were methicillin-sensitive Staphylococcus aureus (MSSA). Phenotype iMLSb was 41 (29.3%), cMLSb phenotype was 37 (26.4%), mecA gene was present in 84 (60%), and none of the samples showed ermA gene positivity.<br>Conclusion: As we know, the presence of the mecA gene is the major evidence for the detection of MRSA isolates. Their presence in low numbers opens the door to search for other mechanisms that may compete with mecA gene in producing resistance phenomenon. The absence of ermA gene in strains S. aureus with iMLSb and cMLSb phenotypes concluded that some other erm gene is responsible for this MLS type of resistance. Due to the frequency of MRSA strains showing the iMLSb phenotype, the use of clindamycin in erythromycin-resistant strains cannot be recommended due to the high possibility of failure in treatment with this antibiotic. J Microbiol Infect Dis 2021; 11(4):6-11.

Key words: Staphylococcus aureus, mecA gen, ermA gene, MRSA, MLSb phenotype


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Shreya Mahesh
Articles by RajKumar Kalyan
Articles by Prashant Gupta
Articles by Sheetal Verma
Articles by Vimala Venkatesh
Articles by Piyush Tripathi
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Shreya Mahesh, RajKumar Kalyan, Prashant Gupta, Sheetal Verma, Vimala Venkatesh, Piyush Tripathi. MecA and ermA Gene Discrepancy from Their Phenotypic Profile in Staphylococcus aureus Isolates. J Microbiol Infect Dis. 2022; 12(1): 6-11 . doi:10.5799/jmid.1085907

Web Style

Shreya Mahesh, RajKumar Kalyan, Prashant Gupta, Sheetal Verma, Vimala Venkatesh, Piyush Tripathi. MecA and ermA Gene Discrepancy from Their Phenotypic Profile in Staphylococcus aureus Isolates. https://www.jmidonline.org/?mno=302657157 [Access: February 03, 2024]. doi:10.5799/jmid.1085907

AMA (American Medical Association) Style

Shreya Mahesh, RajKumar Kalyan, Prashant Gupta, Sheetal Verma, Vimala Venkatesh, Piyush Tripathi. MecA and ermA Gene Discrepancy from Their Phenotypic Profile in Staphylococcus aureus Isolates. J Microbiol Infect Dis. 2022; 12(1): 6-11 . doi:10.5799/jmid.1085907


Vancouver/ICMJE Style

Shreya Mahesh, RajKumar Kalyan, Prashant Gupta, Sheetal Verma, Vimala Venkatesh, Piyush Tripathi. MecA and ermA Gene Discrepancy from Their Phenotypic Profile in Staphylococcus aureus Isolates. J Microbiol Infect Dis. (2022), [cited February 03, 2024]; 12(1): 6-11 . doi:10.5799/jmid.1085907


Harvard Style

Shreya Mahesh, RajKumar Kalyan, Prashant Gupta, Sheetal Verma, Vimala Venkatesh, Piyush Tripathi (2022) MecA and ermA Gene Discrepancy from Their Phenotypic Profile in Staphylococcus aureus Isolates. J Microbiol Infect Dis, 12 (1), 6-11 . doi:10.5799/jmid.1085907


Turabian Style

Shreya Mahesh, RajKumar Kalyan, Prashant Gupta, Sheetal Verma, Vimala Venkatesh, Piyush Tripathi. 2022. MecA and ermA Gene Discrepancy from Their Phenotypic Profile in Staphylococcus aureus Isolates. Journal of Microbiology and Infectious Diseases, 12 (1), 6-11 . doi:10.5799/jmid.1085907


Chicago Style

Shreya Mahesh, RajKumar Kalyan, Prashant Gupta, Sheetal Verma, Vimala Venkatesh, Piyush Tripathi. "MecA and ermA Gene Discrepancy from Their Phenotypic Profile in Staphylococcus aureus Isolates." Journal of Microbiology and Infectious Diseases 12 (2022), 6-11 . doi:10.5799/jmid.1085907


MLA (The Modern Language Association) Style

Shreya Mahesh, RajKumar Kalyan, Prashant Gupta, Sheetal Verma, Vimala Venkatesh, Piyush Tripathi. "MecA and ermA Gene Discrepancy from Their Phenotypic Profile in Staphylococcus aureus Isolates." Journal of Microbiology and Infectious Diseases 12.1 (2022), 6-11 . Print. doi:10.5799/jmid.1085907


APA (American Psychological Association) Style

Shreya Mahesh, RajKumar Kalyan, Prashant Gupta, Sheetal Verma, Vimala Venkatesh, Piyush Tripathi (2022) MecA and ermA Gene Discrepancy from Their Phenotypic Profile in Staphylococcus aureus Isolates. Journal of Microbiology and Infectious Diseases, 12 (1), 6-11 . doi:10.5799/jmid.1085907


Author LoginReviewer LoginAbout PublisherOpen Access PolicyEditorial PoliciesPeer Review PolicyPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyProtection of Research Participants (Statement On Human And Animal Rights)Privacy PolicyCorrections, Retractions & Expressions of ConcernSelf-Archiving PoliciesDigital Archiving & Preservation PoliciesStatement of Informed ConsentAdvertising PolicyTerms of UseLicense InformationCopyright Information

About Journal of Microbiology and Infectious Diseases

Journal of Microbiology and Infectious Diseases (JMID) is an international "peer review" journal in microbiology, infectious diseases, virology, ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes

Editor-in-Chief

Journal of Microbiology and Infectious Diseases

Department of Microbiology and Parasitology 

Faculty of Veterinary Medicine

University of Tripoli

P.O. Box 13662, Tripoli, Libya