E-ISSN 2146-9369 | ISSN 2146-3158
 

Research Article 


Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa.

Cited By:2

Abstract
Objectives:Echinococcosis is a cyclozoonotic disease caused by E. granulosus. The canine mainly the dog represents the definitive host and immunity plays an important role in the control of the disease. This study was aimed to detect Echinococcus granulosus B-antibody for Dot-ELISA and antigen for counter-current immune electrophoresis (CIEP) and bacterial co-agglutination assay (Co-A).<br>Methods: Follow-up of 23 surgical and chemotherapy patients from different hospitals of Khartoum were performed.<br>Results: Results: Sensitivity and specificity for Dot-ELISA was 94.4% and 100%, respectively within seven days to 6 months except for the relapse all or most of the cases were negative for the disease whereas the sensitivity of CIEP and Co-A for Echinococcusgranulosus antigen in patient sera was 77.8% and 94.4% respectively with a specificity of 100% and 75% respectively. Dot-ELISA, showed high positive and negative predictive value (100%, 95.2%), respectively with CIEP (100%, 83.3%) and in Co-A (81%, 94.1%).<br>Conclusion: Conclusion: Therefore, detection of antigen of E. granulosus in the serum of operative, post-operative, or chemotherapeutic treated patient using CIEP and Co-A or detection of antibodies using IgG ELISA to the antigen B-rich fraction in (ELISA) assay is useful for following treated hydatid patients. J Microbiol Infect Dis 2021; 11(2):88-94.

Key words: Echinococcus granulosus, Dot-ELISA, Co-agglutination assay, immune electrophoresis


 
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How to Cite this Article
Pubmed Style

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa. Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. J Microbiol Infect Dis. 2021; 11(2): 88-94 . doi:10.5799/jmid.951600


Web Style

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa. Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. https://www.jmidonline.org/?mno=302657119 [Access: February 03, 2024]. doi:10.5799/jmid.951600


AMA (American Medical Association) Style

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa. Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. J Microbiol Infect Dis. 2021; 11(2): 88-94 . doi:10.5799/jmid.951600



Vancouver/ICMJE Style

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa. Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. J Microbiol Infect Dis. (2021), [cited February 03, 2024]; 11(2): 88-94 . doi:10.5799/jmid.951600



Harvard Style

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa (2021) Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. J Microbiol Infect Dis, 11 (2), 88-94 . doi:10.5799/jmid.951600



Turabian Style

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa. 2021. Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. Journal of Microbiology and Infectious Diseases, 11 (2), 88-94 . doi:10.5799/jmid.951600



Chicago Style

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa. "Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination." Journal of Microbiology and Infectious Diseases 11 (2021), 88-94 . doi:10.5799/jmid.951600



MLA (The Modern Language Association) Style

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa. "Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination." Journal of Microbiology and Infectious Diseases 11.2 (2021), 88-94 . Print. doi:10.5799/jmid.951600



APA (American Psychological Association) Style

Omer Hassan Siddig Elamin, Mai Abdalla Ali, Ibrahim Alhag Almahadi Elgezuli, Mona Alrasheed Bashir, Ivan Kahwa (2021) Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. Journal of Microbiology and Infectious Diseases, 11 (2), 88-94 . doi:10.5799/jmid.951600