E-ISSN 2146-9369 | ISSN 2146-3158
 

Research Article


Molecular detection of Escherichia coli isolated from human, animal and environmental samples targeting mdh gene: A simple one health approach

Urmy Biswas, Abhi Mallick, Surojit Das.

Abstract
Background:
Escherichia coli cause a wide range of infections in humans and animals in hospitals and community associated with multidrug-resistance, and serves as bioindicator of fecal contamination as well as AMR.
Aim:
Early detection of this bioindicator from humans, animals and environment facilitates characterization with respect to AMR and bacterial forensics for effective public health and infection control measurements.
Method:
We investigated a simple and rapid species-specific end-point and real-time PCR assays targeting malate dehydrogenase (mdh) gene to detect Escherichia coli isolated from livestock, human, and environmental water in Midnapore, West Bengal, India in a timely and cost-effective approach.
Results:
Of 126 E. coli isolates, 53, 41 and 32 were cultured from livestock, human and environmental water. The mdh gene was present in 120 (95.2%) isolates, including all environmental isolates. Two (3.8%) animal and four human (9.8%) isolates were discordant between culture and PCR results. The PCR assays produced results in about 90 min with a running cost of US$3-5 per sample.
Conclusion:
Both the PCR assays may be utilized in detection of Escherichia coli depending on the laboratory infrastructure.

Key words: Escherichia coli, Bioindicator, PCR, mdh gene


 
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How to Cite this Article
Pubmed Style

Biswas U, Mallick A, Das S. Molecular detection of Escherichia coli isolated from human, animal and environmental samples targeting mdh gene: A simple one health approach. J Microbiol Infect Dis. 2023; 13(4): 171-174. doi:10.5455/JMID.2023.v13.i4.1


Web Style

Biswas U, Mallick A, Das S. Molecular detection of Escherichia coli isolated from human, animal and environmental samples targeting mdh gene: A simple one health approach. https://www.jmidonline.org/?mno=155274 [Access: February 02, 2024]. doi:10.5455/JMID.2023.v13.i4.1


AMA (American Medical Association) Style

Biswas U, Mallick A, Das S. Molecular detection of Escherichia coli isolated from human, animal and environmental samples targeting mdh gene: A simple one health approach. J Microbiol Infect Dis. 2023; 13(4): 171-174. doi:10.5455/JMID.2023.v13.i4.1



Vancouver/ICMJE Style

Biswas U, Mallick A, Das S. Molecular detection of Escherichia coli isolated from human, animal and environmental samples targeting mdh gene: A simple one health approach. J Microbiol Infect Dis. (2023), [cited February 02, 2024]; 13(4): 171-174. doi:10.5455/JMID.2023.v13.i4.1



Harvard Style

Biswas, U., Mallick, . A. & Das, . S. (2023) Molecular detection of Escherichia coli isolated from human, animal and environmental samples targeting mdh gene: A simple one health approach. J Microbiol Infect Dis, 13 (4), 171-174. doi:10.5455/JMID.2023.v13.i4.1



Turabian Style

Biswas, Urmy, Abhi Mallick, and Surojit Das. 2023. Molecular detection of Escherichia coli isolated from human, animal and environmental samples targeting mdh gene: A simple one health approach. Journal of Microbiology and Infectious Diseases, 13 (4), 171-174. doi:10.5455/JMID.2023.v13.i4.1



Chicago Style

Biswas, Urmy, Abhi Mallick, and Surojit Das. "Molecular detection of Escherichia coli isolated from human, animal and environmental samples targeting mdh gene: A simple one health approach." Journal of Microbiology and Infectious Diseases 13 (2023), 171-174. doi:10.5455/JMID.2023.v13.i4.1



MLA (The Modern Language Association) Style

Biswas, Urmy, Abhi Mallick, and Surojit Das. "Molecular detection of Escherichia coli isolated from human, animal and environmental samples targeting mdh gene: A simple one health approach." Journal of Microbiology and Infectious Diseases 13.4 (2023), 171-174. Print. doi:10.5455/JMID.2023.v13.i4.1



APA (American Psychological Association) Style

Biswas, U., Mallick, . A. & Das, . S. (2023) Molecular detection of Escherichia coli isolated from human, animal and environmental samples targeting mdh gene: A simple one health approach. Journal of Microbiology and Infectious Diseases, 13 (4), 171-174. doi:10.5455/JMID.2023.v13.i4.1