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Abstract - Molecular characterization and antibiogram of Listeria monocytogenes isolated from chicken and mutton of retail markets
Vinay Kumar B.N, Md. Nadeem Fairoze, Madhavaprasad C.B., Nagappa Karabasanavar, Kotresh A.M., Prakash Nadoor, Prashant S. Bagalkote, Shilpa A.G, Barbuddhe S.B., Kitin Kurkure, Sandeep Chaudhary

Vinay Kumar B. N., Nadeem Fairoze, Madhavaprasad C. B., Nagappa Karabasanavar, Kotresh A. M, Prakash Nadoor, Prashant S., Shilpa A. G., S. B. Barbuddhe, Nitin Kurkure, Sandeep Chaudhary

Nagappa Karabasanavar,

Department of Veterinary Public Health & Epidemiology, Veterinary College, Shivamogga, Karnataka, India Email: pub.nag@gmail.com

Molecular characterization and antibiogram of Listeria monocytogenes isolated from chicken and mutton of retail markets

ABSTRACT

Objective: To isolate Listeria monocytogenes from chicken and mutton meat sold at retail outlets and to characterize isolates for virulence determinants.

Methods: Listeria monocytogenes was isolated from chicken and mutton meat samples using ISO: 11290 method. Mul­tiplex PCR was performed for the detection of virulence associated genes.

Results: Out of 198 retail meat samples (72 chicken and 126 mutton) analyzed, Listeria monocytogenes was isolated from 4.5% (8.3% chicken and 2.3% mutton) samples. All the 9 isolates (6 from chicken and 3 from mutton) belonged to 1/2a servar and carried virulence genes viz. haemolysin (hlyA), phosphatidylinositol phospholipase (plcA), actin polym­erization protein (actA) and invasive associative protein p60 (Iap).

Conclusion: L. monocytogenes is an organism of food safety and public health significance, its recovery from the meat sold at retail outlets indicated breach in the quality assurance. J Microbiol Infect Dis 2016;6(2): 65-68

Nagappa Karabasanavar, Department of Veterinary Public Health & Epidemiology, Veterinary College, Shivamogga, Karnataka, India Email: pub.nag@gmail.com Molecular characterization and antibiogram of Listeria monocytogenes isolated from chicken and mutton of retail markets, Vinay Kumar B. N., Nadeem Fairoze, Madhavaprasad C. B., Nagappa Karabasanavar, Kotresh A. M, Prakash Nadoor, Prashant S., Shilpa A. G., S. B. Barbuddhe, Nitin Kurkure, Sandeep Chaudhary J Microbiol Infect Dis 2016;6(2): 65-68 doi: 10.5799/ahinjs.02.2016.02.0218 Volume 06, Number 02 (2016)

Volume 06, Number 02 (2016)