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Abstract - The effects of ultraviolet B beams on programmed cell death activities in Staphylococcus epidermidis
Payam Behzadi, Elham Behzadi, Reza Ranjbar

The effects of ultraviolet B beams on programmed cell death activities in Staphylococcus epidermidis

Payam Behzadi, Elham Behzadi, Reza Ranjbar

Department of Microbiology, College of Basic Sciences, Shahr-e-Qods Branch, Islamic Azadi University, Tehran, Iran

ABSTRACT
Objectives: Bacterial skin diseases caused by drug-resistant Staphylococcus epidermidis are known as a big problem in the field of treating management of infectious diseases. Progression of resistant strains has led to use phototherapy in parallel with pharmacotherapy. In this short survey, we tried to obtain a logic Ultraviolet Radiation protocol to induce the process of programmed cell death in irradiated Staphylococcus epidermidis.
Methods: The samples of Staphylococcus epidermidis were classified in 4 categories. Each plate which is known as a category included well-grown colonies of bacteria. One plate was taken as a control sample and the left three plates were irradiated by UVB (302 nm) in 10 minutes from the distance of 8 cm. The irradiated plates were kept in a dark cell and for 1, 24 and 72 hours respectively. Then, total genomic DNA molecules pertaining to all of the colonies comprising control and irradiated samples were harvested by DNP kit and the extracted DNA molecules were running upon the 1% agarose gel together with ethidium bromide.
Results: Control and irradiated samples were studied for probable changes in their macroscopic, microscopic characteristics and then the DNA pattern relating to each group was detected for any variation including smear or DNA laddering bands. No changes were observed in different bacterial properties. No apoptosis were observed in irradiated samples.
Conclusion: UVB is a strong apoptosis stimulus in organisms. However, authors of the present study strongly reject the apoptotic effect of UVB irradiation in the format of the present protocol. J Microbiol Infect Dis 2015;5(1): 21-24

Key words: In vitro, programmed cell death, Staphylococcus epidermidis, UVB

 J Microbiol Infect Dis 2015; 5(1): 21-24 doi: 10.5799/ahinjs.02.2015.01.0169

Volume 05, Number 01 (2015)

Volume 05, Number 01 (2015)