eISSN : 2146-9369
Creative Commons Lisansı
Journal of Clinical and Experimental Investigations is licenced under the terms of Creative Commons Attribution-Non Commercial4.0 International (CC BY-NC 4.0) License.
Abstract - A case of keratitis caused by Scedosporium apiospermum
Asuman Özkan, Serdar Susever, Zayre Erturan, Meltem Uzun, Nilufer Alparslan, Yasemin Oz, Yıldız Yeğenoğlu

Dematiaceous fungi have been reported among the common etiologic agents of keratitis. We herein report a case of keratitis due to Scedosporium apiospermum identified by conventional methods and confirmed by molecular methods. A 68-year-old male who attended the Ophthalmology Clinic at Istanbul University, Faculty of Medicine Hospital, presented with pain, lacrimation and a ‘foreign body’ sensation in the left eye with six weeks' duration. The samples which were taken from corneal tissue, contact lens and anterior chamber were inoculated on Sabouraud dextrose agar (SDA) and brain heart infusion (BHI) agar. On the third day of incubation, a phaeoid mold was observed on the plate which was inoculated with corneal tissue sample at 27°C. The strain was identified as Scedosporium spp with conventional methods and confirmed as S. apiospermum by a molecular method. Infection was successfully treated with voriconazole eyedrop (1%) after pre-diagnosis. Molecular diagnostic methods may be applied for the definitive diagnosis of the pathogen of fungal keratitis and the selection of the appropriate antifungal agent.

Volume 03, Number 01 (2013)