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Abstract - Role of Gallstones in Typhoid Carriage in Egyptian Patients
Marwa Abdelazim Mansour, Tarik Zaher, Amany Ibrahim, Amira Ahmed, Islam Ibrahim, Ashraf Goda, Nehad Ahmed

Objectives: Salmonella typhi forms biofilm on the surface of gallstones promoting colonization and carrier state. The aims are to detect its frequency in chronic calcular cholecystitis patients, to find out their antibiotic resistance pattern, and also to examine biofilm on the surface of gallstones and detect its correlation to antibiotic susceptibility pattern.

Materials and methods: The study group included 257 patients with chronic calcular cholecystitis. S. typhi was isolated from gall bladder specimens. Antibiotics susceptibility tests of the isolates were done. Biofilm on surface of gallstones were visualized using scanning electron microscopy. Virulence (Vi) antibodies against S. typhi were studied in sera of 257 healthy controls.

Results: 10.9% of patients were chronic typhoid carriers compared to only 3.5% of the controls (OR= 3.37, p=0.002). Multidrug resistance was detected in 35.7% of isolates. S. typhi biofilms on the surface of the 21/28 (75%) gallstones were detected. Ninety percent of the strains that produced maximum amount of biofilm were multidrug-resistant.

Conclusion: Chronic calcular cholecystitis patients are more prone to typhoid carriage due to biofilm formation of S. typhi on the surface of gallstones. J Microbiol Infect Dis 2012; 2(4): 142-149

Volume 02, Number 04 (2012)