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Abstract - Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs
Mustafa Altındiş, Zafer Çetinkaya, Raike Kalaycı, İhsan H. Çiftçi, Alpaslan Arslan, Orhan C. Aktepe

 

Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs

Mustafa Altındiş, Zafer Çetinkaya, Raike Kalaycı, Ihsan H Ciftçi, Alpaslan Arslan, Orhan C. Aktepe

Afyon Kocatepe University, School of Medicine, Department of Medical Microbiology, Afyon, Turkey.

Correspondence: Mustafa Altındiş, MD PhD

Afyon Kocatepe University, School of Medicine, Dept. Medical Microbiology, Afyon, Turkey, Email: maltindis@gmail.com

Received: 25.05.2011, Accepted: 28.06.2011

Copyright © Journal of Microbiology and Infectious Diseases 2011, All rights reserved

 

ABSTRACT

Objectives: The aim of the present study was to evaluate the efficacy (recovery rate, time to detection and Drug Susceptibility Tests –DST- of Mycobacteria-only B460) of new colorimetric medium, Dio-TK and to compare it with routinely used conventional media, Lowenstein Jensen (LJ) and Bactec 460 TB culture system.

Materials and methods: Totally 901 clinic specimens were investigated for assignment of tuberculosis by Ehrlich-Ziehl-Nielsen smear strain method, Lowenstein-Jensen, BACTEC 460TB and Dio-TK medium culture systems.

Results: Nineteen of 901 clinic specimens (2.1%) were positive by any of these methods. 17 (89.5%) of these specimens positive found by smear strain method, 17 (89.5%) by Lowenstein-Jensen, 19 (100%) by BACTEC 460TB and 14 (73.7%) by Dio-TK medium. NAP and Niacin identification tests were applied to Mycobacterium strains. 12 (63.1%) of 19 isolates were identified as M.tuberculosis complex and 7 (36.9%) were identified as Mycobacterium other than tuberculosis (MOTT) bacilli. 10 (83.3%) of 12 M.tuberculosis complex strains were not resistant to any major drug. But one of 2 isolate was resistant to streptomycin and the other one isolate was resistant to both streptomycin and isoniazid.

Conclusion: Our data suggest that some advantages (such as an early detection and differentiation mycobacterium growth from contamination) of the Dio-TK CS over other mycobacterial culture systems make it a practical and rapid system for daily use, and a suitable alternative to other currently available solid media, such as LJ, for detection time of mycobacteria and DST. J Microbiol Infect Dis 2011;1(1):5-9. doi: 10.5799/ahinjs.02.2011.01.0002

Key words: Dio-TK medium, Mycobacterium tuberculosis, detection, resistance

Volume 01, Number 01 (2011)