eISSN : 2146-9369
Creative Commons Lisansı
Journal of Clinical and Experimental Investigations is licenced under the terms of Creative Commons Attribution-Non Commercial4.0 International (CC BY-NC 4.0) License.
Abstract - Plasmid-mediated Colistin Resistance mcr-1 in A Clinical Isolate of Escherichia coli ST19 in Algeria
Selma Bouheraoua, Farida Assaoua, Nora Zourdani, Naima Tahrat, Hassiba Tali Maamar


Plasmid-mediated colistin resistance mcr-1 in a clinical isolate of Escherichia coli ST19 in Algeria


Selma Bouheraoua1,2, Farida Assaous2, Nora Zourdani2, Naima Tahrat2, Hassiba Tali Maamar2

1Université d’Alger, Faculté de Médecine, Département de Pharmacie, Alger, Algeria

2Laboratoire de Bactériologie Médicale et de Surveillance de La Résistance des Bactéries aux Antibiotiques, Institut Pasteur d’Algérie. Route du petit Staoueli. Dely Ibrahim, Algiers, Algeria



We describe the emergence of plasmid-mediated colistin resistance mcr-1 in a clinical isolate of Escherichia coli ST19. The strain was isolated from a urine sample of a 75-year-old male patient. The identification was carried out by an automated system. The antibiogram was performed according to CLSI recommendations. The colistin MIC was determined by broth micro-dilution according to CA-SFM/EUCAST criteria. The detection of mcr-1 was carried out by immunochromatographic test and PCR mcr-1. Genetic transfers and determination of the plasmid incompatibility group were made. The ST was carried out by MLST using the Pasteur scheme.

The strain has a high-level penicillinase resistance phenotype. It was susceptible to amikacin and resistant to gentamicin, tobramycin, fluoroquinolones, tetracycline, chloramphenicol, and cotrimoxazole. The strain was resistant to colistin (MIC ꞊ 4 μg/ml).

The genetic transfer is given a colistin-resistant transconjugant. The plasmid carrying mcr-1 gene belongs to the incompatibility group IncHI2.The strain belongs to ST19. J Microbiol Infect Dis 2019; 10(4):222-224.

Keywords: Colistin resistance, mcr-1, Escherichia coli ST19



Volume 10, Number 04 (2020)