eISSN : 2146-9369
Creative Commons Lisansı
Journal of Clinical and Experimental Investigations is licenced under the terms of Creative Commons Attribution-Non Commercial4.0 International (CC BY-NC 4.0) License.
Abstract - Molecular detection and characterization of Merkle Cell Polyoma virus in Lymphomas among Sudanese patients.
Mohammed A Ibrahim, Khalid A Enan, Abdel Rahim M El Hussein, Mohamed O Mustafa, Isam M Elkhidir

Molecular detection and Characterization of Merkle Cell Polyoma Virus in Lymphomas among Sudanese Patients

Mohammed A Ibrahim1, Khalid A Enan1, Abdel Rahim M El Hussein1, Mohamed O Mustafa1, Bashir Salim2, Isam M Elkhidir3

1Department of Virology, Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan

2Department of Parasitology, Faculty of Veterinary Medicine, University of Khartoum, P.O. Box 32, Khartoum North, Sudan

3Department of Microbiology and Parasitology, Faculty of Medicine, University of Khartoum, Sudan

 

ABSTRACT

Objectives: Lymphoma (Hodgkin and Non-Hodgkin) is one of the most common malignancies in Sudan. Contemporaneous studies from several countries including the United States, Japan and Germany suggested a possible etiological link between Merkle cell polyoma virus (MCPyV) and these lymphomas; however, there are no previous studies that have been reported on this topic in Sudan. This study was applied to investigate the role of this virus in lymphoma patients in Sudan. Objective is to detect and characterize Merkle Cell Polyomavirus in lymphoma patients by using molecular techniques

Method: Adult Sudanese patients (both females and males) diagnosed with lymphoma were included in this study. Blood samples were used for DNA extraction which was then subjected to Real-time PCR to detect MCPyV DNA. Samples, proved positive by Real-time PCR amplification, were sequenced and phylogenetically analyzed.

Results: MCPyV was detected in 16/225 from Sudanese (7.1%) of lymphoma patients of which five were Hodgkin and eleven were Non-Hodgkin. MCPyV sequences in Sudanese patients showed 100% similarity to each other and 100% identity with reference sequences from gene bank.

Conclusion: This study is the first evidence that MCPyV exists in a subset of Sudanese lymphoma patients. MCPyV is unlikely to have direct contribution in the pathogenesis of lymphoma in the majority of Sudanese cases. MCPyV identified from the Sudanese lymphoma subjects may constitute an African lineage and its pathogenicity needs to be investigated in future studies. J Microbiol Infect Dis 2020; 10(1):47-52.

Keywords: Merkle Cell Polyomavirus, Hodgkin Lymphoma, Non-Hodgkin, Real-time PCR, Sudan

Volume 10, Number 01 (2020)