Deficiency in Fat-soluble Vitamins A, D, E in Patients with Pulmonary Multidrug-Resistant Tuberculosis

Lydie Boyvin ^1,2, Bahi Gnogbo Alexis^1,2, Yayé Yapi Guillaume^1,3, Séri Kipré Laurent^1,2, Aké Aya Jeanne Armande^1,2, Djaman Allico Joseph^1,2

¹Laboratory of Biochemical Pharmacodynamics, University Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire

²Clinical & Basic Biochemistry Department, Institut Pasteur, Abidjan, Côte d’Ivoire,

³Biochemistry-Microbiology Department, University Jean Lourougnon Guédé, Abidjan, Côte d’Ivoire,

ABSTRACT

Objective: The treatment management of Multidrug-Resistant Tuberculosis (MDR-TB) is a major global public health problem. The development of this form of tuberculosis increases immune deficiency and the production of free radicals in the body. Micronutrients, especially fat-soluble vitamins A, D, and E, play an essential role in the immune system by protecting and renewing cells. The objective of this study is to determine the profiles of vitamins A, D, and E in order to evaluate the performance of the immune defenses of MDR-TB under second-line anti-TB treatment.

Methods: The analysis of vitamins A, D and E was carried out using an HPLC chain, in isocratic mode by UV-Visible detection after prior extraction of the lipid fraction from the serum in the hexane protected away from light.

Results: MDR-TB showed a significant decrease in the concentration of vitamins A, D, and E (p˂0.05) with high reduction levels of 80%, 40% and 50%, respectively.

Conclusion: The persistence of this deficit after six months of TB treatment highlights the need for corrective measures to be taken, such as the supplementation of vitamins A, D, and E. J Microbiol Infect Dis 2020; 10(4): 199-207.

Keywords: Micronutrients, Multidrug-resistant, Tuberculosis, HPLC, Côte d’Ivoire