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Abstract - Group B streptococcus detection in China: comparison of different screening methods and different sampling sites
Xi Wang, Yingna Song, Liangkun Ma, Juntao Liu, Yingchun Xu, Jie Yi

Group B streptococcus detection in China: comparison of different screening methods and different sampling sites

Xi Wang1, Yingna Song1, Liangkun Ma1, Juntao Liu1, Yingchun Xu2, Jie Yi2

1Department of Obstetrics and Gynecology, 2Laboratory Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, PR China

ABSTRACT

Objectives: To evaluate the use of real-time polymerase chain reaction (RT-PCR) and bacterial culture methods to detect group B streptococcus (GBS) in Chinese pregnant women in the third trimester; to separately assess the prevalence of rectal and vaginal GBS colonization ; and to determine the antimicrobial susceptibility pattern of the isolates.

Methodology: Samples were collected from 505 women at 35 and 37 weeks gestation at the Peking Union Medical College Hospital. Bacterial culture and RT-PCR were performed. Antimicrobial susceptibility to commonly used antibiotics was also analyzed.

Results: The overall GBS colonization rate was 7.5%. The colonization rate, sensitivity, and negative predictive value of the bacterial culture method were 2.8%, 36.8%, and 95.1%, respectively, and these values were 7.3%, 97.4%, and 99.8%, respectively, for PCR (p<0.001). The GBS colonization rate of the rectum (6.7%) was higher than that of the vagina (2.8%) (p=0.005). Antimicrobial susceptibility testing showed that 100% were sensitive to penicillin, cephalosporin and vancomycin.

Conclusions: RT-PCR was found to be a rapid and sensitive test for the detection of GBS colonization in Chinese pregnant women. Rectal swabbing was also important for detecting GBS colonization. β-lactams are the first-line antibiotics used for the treatment of GBS. J Microbiol Infect Dis 2016;6(4): 179-183

Key words: Group B streptococcus; Real-time polymerase chain reaction; Sampling sites

Volume 06, Number 04 (2016)