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Abstract - Evaluating correlation of the native Inaba strain with the dominant isolated strains in outbreaks occurred in Iran at 2013 by Pulsed Field Gel Electrophoresis
Massoud - Hajia, Alireza - Dolatyar, Marjan Rahnami Farzami, Mohsen - Imani, Roghieh - Saburian, Mohamad - Rahbar

Evaluating correlation of the native Inaba strain with the dominant isolated strains in outbreaks occurred in Iran at 2013 by Pulsed Field Gel Electrophoresis

Massoud Hajia1, Alireza Dolatyar2, Marjan Rahnami Farzami3, Mohsen Imani4, Roghieh Saburian4, Mohamad Rahbar1

1 Department of Molecular Biology, Research center of Health Reference Laboratories, Ministry of Health and Medical Education, Tehran, Iran

2 Research center of Health Reference Laboratories, Ministry of Health and Medical Education, Tehran, Iran

3 Research center of Health Reference Laboratories, Ministry of Health and Medical Education, Tehran, Iran

4 Center for Communicable Diseases Control. Ministry of Health and Medical Education, Iran

ABSTRACT

Objectives: The aim of this study was to analyze the isolated cholera strains at outbreak 2013 for studying the their similarity and compare their homology in order to find out the route of infection either emerge from abroad or reemerge from inside native strains.

Methods: All diagnosed V. cholerae isolates were entered to the study after re-identification at referral laboratory of Health Ministry based on standard procedures. These specimens were examined for specific serogroups by O1 polyvalent and Ogawa/Inaba nonspecific antisera and tested by MIC Test Strip Method against Ciprofloxacin, Nalidixic Acid, Cefixime, Ampicillin, Tetracycline, Trimethoprim-Sulfamethoxazole, and Erythromycin.

Results: A total of 257 clinical Vibrio cholerae was isolated in an outbreak of Iran at 2013. The dominant causative type was Inaba. In Antibiotic susceptibility test isolates were 100% resistant to all except Erythromycin that just 23% of strains were sensitive. Homology of isolates was investigated through genotyping by PFGE method and their clonality was compared with previous isolated Iranian native strain. Overall 92% of analyzed strains showed a homolog pattern. These strains were located in 8 clusters. Although isolated strains at 2011 had 80 % homology with recent isolates, located in complete distinct cluster than all strains isolated at 2013. PFGE analysis revealed no dissimilarity between those stains resistant and sensitive to Erythromycin.

Conclusion: This study confirmed that isolated Inaba strains at 2013 had different clonality pattern in PFGE than previously identified, suggested have foreign route from the neighboring countries. J Microbiol Infect Dis 2016;6(4): 184-189

Key words: Vibrio cholera, Outbreak, Inaba, PFGE

Volume 06, Number 04 (2016)